2D and 3D Cellular Screening Models and AI Guided Analysis, Volume 204 explores the development, application, and comparative benefits of 2D monolayer cultures and 3D organoids, spheroids, and tissue-engineered constructs for biomedical research and drug discovery. Chapters in this new release include High throughput CellTiter-glo viability assessment, Assessing viability of cancer cell spheroids in 3D drug screening, 3D killing assay of cancer spheroids by cytotoxic T lymphocytes in anchored microfluidic droplets, Use of micropatterning for 3D cell culture, Use of microfluidic chip devices for assessing the effect of radiation on 3D cell culture models, and much more.Additional chapters cover A 3D model for assessing regulated cell death modalities, YOLO detection model for Cell Viability assessment, Bioinformatics and AI/ML approaches using multi-omics data to accelerate diagnosis and delivery of precision care, Automated image analysis and machine learning for the assessment of mitochondrial membrane potential, Assessing cancer cell heterogeneity in patient-derived tumoroids, Assessment of lysosomal drug sequestration and release using fluorescence microscopy, Assessment of T cell toxicity in ex-vivo models of cancer, Three-dimensional Organoid Screening using Leica Thunder Microscope, Using vascularized organoids-on-chip for assessing systemic drug delivery, and many more interesting and timely topics. - Includes detailed methodologies and protocols for developing both 2D monolayer and 3D spheroid/organoid cellular screening models - Presents in-depth comparisons of the advantages, limitations, and applications of 2D vs. 3D cellular models in research and drug discovery - Provides high-throughput and high-content screening methods for assessing cellular responses