This volume comprehensively covers techniques that use activity-based proteomics to investigate biological functions of proteins such as lipolytic enzymes, serine hydrolases, chitinases, and glycoside hydrolases. The chapters in this book cover a wide range of protocols and an overview on the application of activity-based proteomics profiling to study deISGylating enzymes, serine hydrolases, monooxygenases, ubiquitin E3 ligases, and reactive cysteine in various organisms, including bacteria, viruses, and mammalian cells and tissues. Furthermore, this book provides updated, comprehensive protocols for utilizing activity-based proteomics profiling to discover new enzymes, detect novel modifications, and identify new drug therapy targets. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on trouble3.shooting and avoiding known pitfalls. Cutting-edge and comprehensive, Activity-Based Proteomics: Methods and Protocols is a valuable resource for all researchers who are interested in applying the latest technologies in this advancing field.