Energy harvesting is the conversion of unused or wasted energy in the ambient environment into useful electrical energy. It can be used to power small electronic systems such as wireless sensors and is beginning to enable the widespread and maintenance-free deployment of Internet of Things (IoT) technology. This Special Issue is a collection of the latest developments in both fundamental research and system-level integration. This Special Issue features two review papers, covering two of the hottest research topics in the area of energy harvesting: 3D-printed energy harvesting and triboelectric nanogenerators (TENGs). These papers provide a comprehensive survey of their respective research area, highlight the advantages of the technologies and point out challenges in future development. They are must-read papers for those who are active in these areas. This Special Issue also includes ten research papers covering a wide range of energy-harvesting techniques, including electromagnetic and piezoelectric wideband vibration, wind, current-carrying conductors, thermoelectric and solar energy harvesting, etc. Not only are the foundations of these novel energy-harvesting techniques investigated, but the numerical models, power-conditioning circuitry and real-world applications of these novel energy harvesting techniques are also presented.