This brief presents recent developments and new methodologies in the area of petroleum geochemistry, including innovative unconventional analytical methods such as Raman, NMR and XPS spectroscopy that are based on advanced analytical chemistry and recently developed instrumentation. It discusses delineating mechanisms for petroleum generation, expulsion, migration and accumulation in reservoirs from the source rock. This brief provides data, illustrations and detailed explanation of each method discussed, as well as data collection, interpretation of the results and intercorrelated characteristics of different case studies from a variety of samples. Geologists, engineers, and graduate students interested in the petroleum industry will all find the book valuable.