This book covers all the aspects of surface enhanced Raman Spectroscopy (SERS) including a brief history of the discovery of SERS and development of the field, basic principles and enhancement mechanism of SERS. It covers first generation (plasmonic nanomaterials) /second generation (plasmonic hot spots)/third generation (via plasmonic engineering) substrate nanomaterials that are developed for SERS, single molecule SERS and designed SERS probes for ultrasensitive detection applications. It further discusses different SERS detection platforms, highlight the issues of poor signal reproducibility and how to minimize it. Features: Provides understanding on the role of electromagnetic hot spots in surface enhanced Raman Spectroscopy. Reviews all types of surface enhanced Raman Spectroscopy substrates. Covers surface enhanced Raman Spectroscopy-based detection platforms and signal reproducibility issues. Includes development of single molecule detection via surface enhanced Raman Spectroscopy. Discusses chemical analysis, biomedical application, and all other related applications. This book is aimed at graduate students and researchers in spectroscopy, materials science, and engineering.