Environmental analysis is one of the most relevant scientific fields in chemistry that contributes to the monitoring of environmental pollution. Green methodologies should be developed and applied in order to investigate the impact of pollution on the environment. The scope of this Special Issue was to describe the state of the art in both analysis and sample pretreatment with regard to the demands of green perspectives for the examination of the current status of environmental pollution. Analytical scientists were invited to present new strategies to fulfill all the requirements for reductions in waste, energy consumption, the use of solvents, etc. The Special Issue includes fourteen papers: 11 original articles, 2 review articles and 1 perspective.