There is a pervasive need for analytical separations in many fields of science and in many different industries. Analytical measurements are vital to understand processes and materials, to develop or improve products and to protect the health of individuals and entire ecosystems. Separations are needed in many situations because most samples are (complex) mixtures. Thus, scientists in academia and industry need analytical separations. This book teaches (almost) all the important aspects of analytical separation science to students and practicing analysts at various points in their careers. For those new to separation science, it features basic modules in which fundamental concepts and analytical practice are described. Master modules provide more in-depth treatments intended for graduate students. Advanced modules bring the reader to the current frontiers of separation science and encourage further reading. Emphasis is on the main analytical separation techniques of gas chromatography, liquid chromatography and capillary electrophoresis, but due attention is paid to a number of other methods and to sample preparation. Knowledge on statistics and chemometrics is provided, so that separation scientists can obtain relevant information from analytical data. Finally, the book describes how a sound understanding can be used to successfully develop optimal separation methods.