Handbook of Practical Pharmacology I serves as a vital bridge between theoretical pharmacology and laboratory application, equipping learners with the experimental skills essential to understanding drug actions through hands-on animal studies. A comprehensive, curriculum-based guide designed for second-year B. Pharm. students, fully aligned with the Pharmacy Council of Indias 2014 regulations. The book introduces students to fundamental laboratory concepts, animal handling as per CCSEA guidelines, drug administration routes, and experimental models like Rotarod, Actophotometer, and rabbit eye studies. It explores pharmacodynamic responses to various classes of drugs, including hepatic enzyme inducers, skeletal muscle relaxants, anticonvulsants, and anxiolytics. Each chapter combines clarity, precision, and ethical awareness, ensuring reproducibility and adherence to professional standards. Serving both as a laboratory manual and a learning companion, this handbook enhances analytical thinking and experimental competence, helping students gain a deeper, practice-based understanding of pharmacology. Key Features Aligns with the PCI 2014 syllabus for second-year B. Pharm. students. Explains fundamental experimental pharmacology concepts and instrumentation. Demonstrates animal handling and drug administration techniques per CCSEA guidelines. Guides pharmacodynamic testing using established models like Rotarod, Actophotometer, and MES. Promotes laboratory competence, ethical compliance, and analytical reasoning in pharmacology