This book constitutes the proceedings of the 16th International Conference on Management of Digital EcoSystems, MEDES 2024, held in Naples, Italy, during November 2024.  The 23 full papers and 10 Short papers included in this book were carefully reviewed and selected from 65 submissions. They were organized in topical sections as follows: Energy and Environment; Recommendation Systems; Social Networks and Emotion Recognition; Medical and Health Issues; Cybersecurity and Privacy; Data Mining Operations; Parallel and Distributed Systems; Network Aspects, and Applications.