I believe that this book will be helpful for the Undergraduate students, research students, and researchers who would like to study and innovate in their own study and research areas related to this topics. This book highlights the plant cell and tissue culture, cell regeneration and stem cell. Fundamental ideas and many research data are included in this book. Cell suspension, protoplast culture, organogenesis and somatic embryogenesis are described well. In vitro banana, broccoli, pineapple and hibiscus sp.cultures discussed. Stem cell culture from muscle, skeletal and other cells is mentioned. In addition to that Plant cell and DNA cloning, rDNA, T-DNA, DNA trasformation, molecular cloning related to cell culture are described mentioning different research data. Meristem culture, shoot tip culture and leaf culture, embryo culture, anther culture to generate callus as well as whole plant are discussed. Different hormones effects like BAP, IBA, NAA and GA3 to regenerate the cell and tissue are described well.