This book constitutes the proceedings of the 16th Asian Conference on Intelligent Information and Database Systems, ACIIDS 2024, held in Ras Al Khaimah, UAE, during April 15-18, 2024.The 58 full papers are presented in this book were carefully reviewed and selected from 251 submissions. They are organized in topical sections as follows: Part One: AI-driven Cybersecurity Solutions; AI-driven Medical Analytics; Computational Intelligence; and Data Modelling and Information Systems.Part Two: Image and Video Processing; Prediction and Recommendation Systems; and Text, Speech and Natural Language Processing.