This volume presents comprehensive laboratory protocols that have been used to generate Th9 cells, both in vitro and in vivo. The techniques described inTh9 Cells: Methods and Protocolsstudy the role of Th9 cells in different inflammatory disease models, including allergic inflammation model, parasite model, tumor model, and EAE and IBD model. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Thorough and comprehensive,Th9 Cells: Methods and Protocolsis a valuable resource for scientists in this field and will provide them with techniques to generate Th9 cells for specific downstream events.