This volume provides methods on the role of TLRs in immune responses and on the various approaches targeting different TLRs in cancer, autoimmunity and in vaccine development. Written in the highly successful  Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge,  Toll-Like Receptors: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.