progress in understanding these mechanisms, their realization in differentchemicalstructuresisoverviewed. vii viii Preface Demonstratingtheprogressinaninterdisciplinary?eldofresearchanddev- opment, thisbookisprimarilyaddressedtospecialistswithdifferentbackground- physicists, organic and analytical chemists, and photochemists - to those who developandapplynew?uorescencereporters. Itwillalsobeusefultospecialists inbioanalysisandbiomedicaldiagnostics. Kyiv, Ukraine AlexanderP. Demchenko June2010 Contents PartI GeneralAspects NanocrystalsandNanoparticlesVersusMolecularFluorescent LabelsasReportersforBioanalysisandtheLifeSciences: ACriticalComparison . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 UteResch-Genger, MarkusGrabolle, RolandNitschke, andThomasNann OptimizationoftheCouplingofTargetRecognition andSignalGeneration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41 AnaB. Descalzo, ShengchaoZhu, TobiasFischer, andKnutRurack CollectiveEffectsIn?uencingFluorescenceEmission . . . . . . . . . . . . . . . . . . . . . 107 AlexanderP. Demchenko PartII EncapsulatedDyesandSupramolecularConstructions FluorescentJ-AggregatesandTheirBiologicalApplications . . . . . . . . . . . . . 135 MykhayloYu. LosytskyyandValeriyM. Yashchuk Conjugates, Complexes, andInterlockedSystems BasedonSquarainesandCyanines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 159 LeonidD. Patsenker, AnatoliyL. Tatarets, OleksiiP. Klochko, andEwaldA. Terpetschnig PartIII Dye-DopedNanoparticlesandDendrimers Dye-DopedPolymericParticlesforSensingandImaging . . . . . . . . . . . . . . . . 193 SergeyM. Borisov, TorstenMayr, Gu¨nterMistlberger, andIngoKlimant ix x Contents Silica-BasedNanoparticles:DesignandProperties . . . . . . . . . . . . . . . . . . . . . . . 229 SongLiang, CarrieL. John, ShupingXu, JiaoChen, YuhuiJin, QuanYuan, WeihongTan, andJuliaX. Zhao LuminescentDendrimersasLigandsandSensors ofMetalIons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 253 GiacomoBergamini, EnricoMarchi, andPaolaCeroni ProspectsforOrganicDyeNanoparticles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 285 HiroshiYao PartIV LuminescentMetalNanoclusters Few-AtomSilverClustersasFluorescentReporters . . . . . . . . . . . . . . . . . . . . . . 307 IsabelD?´ezandRobinH. A. Ras LuminescentQuantumClustersofGoldasBio-Labels . . . . . . . . . . . . . . . . . . . 333 M. A. HabeebMuhammedandT. Pradeep PartV ConjugatedPolymers Structure, EmissiveProperties, andReportingAbilities ofConjugatedPolymers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 357 MaryA. Reppy OpticalReportingbyConjugatedPolymers viaConformationalChanges . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 389 RozalynA. SimonandK. PeterR. Nilsson FluorescenceReportingBasedonFRETBetweenConjugated PolyelectrolyteandOrganicDyeforBiosensorApplications . . . . . . . . . . . . 417 Kan-YiPuandBinLiu Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 455 PartI GeneralAspects NanocrystalsandNanoparticlesVersus MolecularFluorescentLabelsasReporters forBioanalysisandtheLifeSciences: ACriticalComparison UteResch-Genger, MarkusGrabolle, RolandNitschke, andThomasNann Abstract At the core of photoluminescence techniques are suitable ?uorescent labels and reporters, the spectroscopic properties of which control the limit of detection, thedynamicrange, andthepotentialformultiplexing. | Advanced Fluorescence Reporters in Chemistry and Biology II by Alexander P. Demchenko, Hardcover | Indigo Chapters