Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC): Methods andProtocolsprovides a synopsis of a large array of different SILAC methods by presenting a set of protocols that have been established by renowned scientists and their working groups. These include methods and protocols for the labeling of various model organisms as well as advanced strategies relying on SILAC, e. g. for the analysis of protein interactions, the mapping of posttranslational modifications or the characterization of subcellular proteomes. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC):Methods and Protocolswill serve students and experienced scientists alike as a valuable reference of how to make use of the SILAC technology for their own research. | Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) by Bettina Warscheid, Hardcover | Indigo Chapters